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Nexcelom Bioscience celígo imaging cell cytometer
Celígo Imaging Cell Cytometer, supplied by Nexcelom Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cel%C3%ADgo+imaging+cell+cytometer/celigo+image+cytometer/pm31526854-89-15-20
Average 90 stars, based on 1 article reviews
celígo imaging cell cytometer - by Bioz Stars, 2026-09
90/100 stars

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Incubation:

Article Title: Reprogramming AA catabolism in CHO cells with CRISPR/Cas9 genome editing improves cell growth and reduces byproduct secretion.
Article Snippet: In brief, a dye master mix, containing 5 202 μg/mL Hoechst-33342 (Life Technologies), for staining of total cell population, and 0.4 203 μg/mL propidium iodide (Life Technologies), for staining of dead cells, was prepared in 204 CD-CHO medium supplemented with 8 mM L-glutamine, and was transferred to a 96-well 205 optical-bottom microplate (Greiner Bio-One, Frickenhausen, Germany) containing 3 μl of 206 cell suspension in a total volume of 200 μL per well. .. After incubation at room temperature, 207 the cells were imaged using the appropriate channels in Celígo Imaging Cell Cytometer 208 (Nexcelom Bioscience, MA, USA). .. Culture supernatants were analyzed for glucose, 209 lactate, glutamine, glutamate and ammonium using BioProfile 400 Plus (Nova Biomedical, 210 Waltham, MA, USA) and for AAs as described in section 2.6.

Imaging:

Article Title: Reprogramming AA catabolism in CHO cells with CRISPR/Cas9 genome editing improves cell growth and reduces byproduct secretion.
Article Snippet: In brief, a dye master mix, containing 5 202 μg/mL Hoechst-33342 (Life Technologies), for staining of total cell population, and 0.4 203 μg/mL propidium iodide (Life Technologies), for staining of dead cells, was prepared in 204 CD-CHO medium supplemented with 8 mM L-glutamine, and was transferred to a 96-well 205 optical-bottom microplate (Greiner Bio-One, Frickenhausen, Germany) containing 3 μl of 206 cell suspension in a total volume of 200 μL per well. .. After incubation at room temperature, 207 the cells were imaged using the appropriate channels in Celígo Imaging Cell Cytometer 208 (Nexcelom Bioscience, MA, USA). .. Culture supernatants were analyzed for glucose, 209 lactate, glutamine, glutamate and ammonium using BioProfile 400 Plus (Nova Biomedical, 210 Waltham, MA, USA) and for AAs as described in section 2.6.

Article Title: Using Titer and Titer Normalized to Confluence Are Complementary Strategies for Obtaining Chinese Hamster Ovary Cell Lines with High Volumetric Productivity of Etanercept.
Article Snippet: .. The day after, all clones were sub-confluent and the confluence (the percentage of the well covered by cells) and the number of cells in each well was estimated by image cytometry analysis using the Celígo Imaging Cell Cytometer (Nexcelom Bioscience, MA) with the labelfree, bright field confluence or direct cell counting application, respectively. ..

Cytometry:

Article Title: Reprogramming AA catabolism in CHO cells with CRISPR/Cas9 genome editing improves cell growth and reduces byproduct secretion.
Article Snippet: In brief, a dye master mix, containing 5 202 μg/mL Hoechst-33342 (Life Technologies), for staining of total cell population, and 0.4 203 μg/mL propidium iodide (Life Technologies), for staining of dead cells, was prepared in 204 CD-CHO medium supplemented with 8 mM L-glutamine, and was transferred to a 96-well 205 optical-bottom microplate (Greiner Bio-One, Frickenhausen, Germany) containing 3 μl of 206 cell suspension in a total volume of 200 μL per well. .. After incubation at room temperature, 207 the cells were imaged using the appropriate channels in Celígo Imaging Cell Cytometer 208 (Nexcelom Bioscience, MA, USA). .. Culture supernatants were analyzed for glucose, 209 lactate, glutamine, glutamate and ammonium using BioProfile 400 Plus (Nova Biomedical, 210 Waltham, MA, USA) and for AAs as described in section 2.6.

Article Title: Using Titer and Titer Normalized to Confluence Are Complementary Strategies for Obtaining Chinese Hamster Ovary Cell Lines with High Volumetric Productivity of Etanercept.
Article Snippet: .. The day after, all clones were sub-confluent and the confluence (the percentage of the well covered by cells) and the number of cells in each well was estimated by image cytometry analysis using the Celígo Imaging Cell Cytometer (Nexcelom Bioscience, MA) with the labelfree, bright field confluence or direct cell counting application, respectively. ..

Clone Assay:

Article Title: Using Titer and Titer Normalized to Confluence Are Complementary Strategies for Obtaining Chinese Hamster Ovary Cell Lines with High Volumetric Productivity of Etanercept.
Article Snippet: .. The day after, all clones were sub-confluent and the confluence (the percentage of the well covered by cells) and the number of cells in each well was estimated by image cytometry analysis using the Celígo Imaging Cell Cytometer (Nexcelom Bioscience, MA) with the labelfree, bright field confluence or direct cell counting application, respectively. ..

Cell Counting:

Article Title: Using Titer and Titer Normalized to Confluence Are Complementary Strategies for Obtaining Chinese Hamster Ovary Cell Lines with High Volumetric Productivity of Etanercept.
Article Snippet: .. The day after, all clones were sub-confluent and the confluence (the percentage of the well covered by cells) and the number of cells in each well was estimated by image cytometry analysis using the Celígo Imaging Cell Cytometer (Nexcelom Bioscience, MA) with the labelfree, bright field confluence or direct cell counting application, respectively. ..



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Nexcelom Bioscience celígo imaging cell cytometer
Celígo Imaging Cell Cytometer, supplied by Nexcelom Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cel%C3%ADgo+imaging+cell+cytometer/celigo+image+cytometer/pm31526854-89-15-20
Average 90 stars, based on 1 article reviews
celígo imaging cell cytometer - by Bioz Stars, 2026-09
90/100 stars
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